显微镜百科
奥林巴斯显微镜的荧光数据表
所属分类:显微镜百科 点击次数:271 发布日期:2022-07-02
大家好,这里是老上光显微镜知识课堂,在这里你可以学到所有关于显微镜知识,好的,请看下面文章: 作为一个指南,激光共聚焦显微镜和广角荧光显微镜荧光团,下面列出了很多常用的荧光染料的表,与各自的峰值吸收和发射波长的激光光源和建议。虽然作者承担的数据发表在各种可靠准确的报告责任,有几个注意事项必须考虑。荧光染料对环境敏感的和不同的结果将与不同的溶剂和应用得到的。在文献回顾,一个经常会发现相同的荧光色素提供不同的数据。还有,在许多情况下,一个荧光染料的几个子品种。
该手册是荧光探针和化学品的研究,由Molecular Probes, Inc.发表,在俄勒冈州尤金市,是关于荧光染料信息的丰富来源。此外,请参照Omega光学和色度技术。
荧光染料的吸收峰值和发射波长
荧光染料
吸收波长
发射波长
Suggested
Laser
Acid Fuchsin
540
630
Green He-Ne
ACMA (Aminochloromethoxyacridine)
430
474
Blue Diode
Acridine Orange
(Bound to DNA)
502
526
Argon-Ion
Acridine Red
455-600
560-680
Argon-Ion
Acridine Yellow
470
550
Argon-Ion
Acriflavin
436
520
Blue Diode
AFA (Acriflavin Feulgen SITSA)
355-425
460
Blue Diode
Alexa Fluor 350
346
442
Helium-Cadmium
Alexa Fluor 405
401
421
Blue Diode
Alexa Fluor 430
433
541
Blue Diode
Alexa Fluor 488
495
519
Argon-Ion
Alexa Fluor 500
503
525
Argon-Ion
Alexa Fluor 514
518
540
Argon-Ion
Alexa Fluor 532
532
553
Krypton-Argon
Alexa Fluor 546
556
573
Green He-Ne
Alexa Fluor 555
555
565
Krypton-Argon
Alexa Fluor 568
578
603
Krypton-Argon
Alexa Fluor 594
590
617
Orange He-Ne
Alexa Fluor 610
612
628
Orange He-Ne
Alexa Fluor 633
632
647
Red He-Ne
Alexa Fluor 647
650
665
Krypton-Argon
Alexa Fluor 660
663
690
Red Diode
Alexa Fluor 680
679
702
Red Diode
Alizarin Complexon
530-560
580
Green He-Ne
Alizarin Red
530-560
580
Green He-Ne
AMCA
350
445
Helium-Cadmium
Aminoactinomycin D
555
655
Krypton-Argon
6-Aminoquinoline
355
550
Helium-Cadmium
Aniline Blue
370
509
Argon-Ion
ANS (Anilinonaphthalene Sulfonic Acid)
385
485
Blue Diode
Anthroyl Stearate
361-381
446
Argon-Ion
APC (Allophycocyanin)
650
661
Krypton-Argon
Astrazon Brilliant Red 4G
500
585
Argon-Ion
Astrazon Orange R
470
540
Argon-Ion
Astrazon Red 6B
520
595
Argon-Ion
Astrazon Yellow 7 GLL
450
480
Argon-Ion
Atabrine
436
490
Blue Diode
ATTO-TAG CBQ
465
560
Argon-Ion
ATTO-TAG FQ
486
591
Argon-Ion
Auramine O
460
550
Argon-Ion
Aurophosphine
450-490
515
Argon-Ion
Aurophosphine G
450
580
Argon-Ion
BAO 9-(Bisaminophenyloxadiazole)
365
395
Argon-Ion
BCECF
505
530
Argon-Ion
Berberine Sulphate
430
550
Blue Diode
Bisbenzamide
360
600-610
Helium-Cadmium
BOBO-1, BO-PRO-1
462
481
Argon-Ion
BOBO-3, BO-PRO-3
570
604
Krypton-Argon
Blancophor FFG Solution
390
470
Blue Diode
Blancophor SV
370
435
Argon-Ion
BODIPY FL
503
512
Argon-Ion
BODIPY TMR
542
574
Green He-Ne
BODIPY TR
589
617
Yellow He-Ne
BODIPY 492/515
490
515
Argon-Ion
BODIPY 493/503
500
506
Argon-Ion
BODIPY 500/510
509
515
Argon-Ion
BODIPY 505/515
502
510
Argon-Ion
BODIPY 530/550
533
550
Krypton-Argon
BODIPY 558/568
558
568
Krypton-Argon
BODIPY 564/570
564
570
Krypton-Argon
BODIPY 581/591
582
590
Yellow He-Ne
BODIPY 630/650
625
640
Red He-Ne
BODIPY 650/665
647
665
Red He-Ne
BODIPY 665/676
665
676
Red Diode
Brilliant Sulphoflavin FF
430
520
Blue Diode
BTC (Benzothiazole Calcium Indicator)
401, 464
529
Blue Diode
Calcein
494
517
Argon-Ion
Calcein Blue
370
435
Argon-Ion
Calcium Crimson
590
611
Yellow He-Ne
Calcium Green
505
532
Argon-Ion
Calcium Orange
549
576
Green He-Ne
Calcofluor (RW Solution)
370
440
Argon-Ion
Calcofluor White
440
500-520
Blue Diode
Calcofluor White (ABT Solution)
380
475
Argon-Ion
Calcofluor White (Standard Solution)
365
435
Argon-Ion
Carbocyanine LDL
520
560
Argon-Ion
5-(and 6-)-Carboxy SNARF-1 Indicator
548 (Low pH)
576 (High pH)
587 (Low pH)
635 (High pH)
Krypton-Argon
5-Carboxynaphthofluorescein
598
668
Yellow He-Ne
6-Carboxyrhodamine 6G
525
555
Argon-Ion
Cascade Blue
400
425
Blue Diode
Cascade Yellow
402
545
Blue Diode
Catecholamine
410
470
Blue Diode
CCF2 (GeneBLAzer)
402
520
Blue Diode
CellTracker Blue
353
466
Helium-Cadmium
CellTracker Green BODIPY
522
529
Argon-Ion
CFDA (Carboxyfluorescein Diacetate)
495
520
Argon-Ion
Chinacrine
450-490
515
Argon-Ion
Chromomycin A3
450
570
Argon-Ion
CL-NERF
504 (Low pH)
514 (High pH)
587 (Low pH)
540 (High pH)
Argon-Ion
Coriphosphine O
460
575
Argon-Ion
Coumarin-Phalloidin
387
470
Blue Diode
CPM (Coumarin-Maleimide)
385
465
Blue Diode
CTC Formazan
450
630
Argon-Ion
Cy2
489
506
Argon-Ion
Cy3
548
562
Green He-Ne
Cy3.1.8
554
568
Krypton-Argon
Cy3.5
581
596
Krypton-Argon
Cy5
650
670
Krypton-Argon
Cy5.1.8
649
666
Krypton-Argon
Cy5.5
675
695
Red Diode
Cy7
710
805
Near-Infrared
DANS
340
525
Helium-Cadmium
DANSA
340-380
430
Helium-Cadmium
Dansyl Cadaverine
335
518
Helium-Cadmium
Dansyl Chloride
335
500
Helium-Cadmium
Dansyl Hydrazine
336
531
Helium-Cadmium
DAO
(Diaminophenyloxydiazole)
280
460
Helium-Cadmium
DAPI
350
470
Helium-Cadmium
Dapoxyl
373
574
Argon-Ion
Di-4-ANEPPS
496
705
Argon-Ion
Di-8-ANEPPS
488
605
Argon-Ion
DiA
456
590
Argon-Ion
DiD (DilC18(5))
644
665
Krypton-Argon
DIDS
341
414
Helium-Cadmium
DiI-C18(3)
549
565
Green He-Ne
Dimethylamino-5-
Sulphonic Acid
310-370
520
Helium-Cadmium
DiO-C18(3)
484
501
Argon-Ion
Diphenyl Brilliant Flavine 7GFF
430
520
Blue Diode
DM-NERF
497 (Low pH)
510 (High pH)
527 (Low pH)
536 (High pH)
Argon-Ion
DPH (Diphenylhexatriene)
351
430
Helium-Cadmium
Dopamine
340
490-520
Helium-Cadmium
DsRFP (Ds Red Fluorescent Protein)
558
583
Green He-Ne
ELF-97 Alcohol
345
530
Helium-Cadmium
EBFP (Enhanced Blue FP)
380
440
Blue Diode
ECFP (Enhanced Cyan FP)
433, 453
475, 501
Blue Diode
EGFP (Enhanced GFP)
488
507
Argon-Ion
ERTracker Blue/White DPX
374
575
Argon-Ion
EYFP (Enhanced Yellow FP)
513
527
Argon-Ion
Eosin
525
545
Argon-Ion
Erythrosin Isothiocyanate
530
558
Argon-Ion
Ethidium Bromide
510
595
Argon-Ion
Ethidium Homodimer
528
617
Argon-Ion
Euchrysin
430
540
Blue Diode
Evans Blue
550
610
Green He-Ne
FAM (5-Carboxyfluorescein)
492
518
Argon-Ion
Fast Blue
360
410
Helium-Cadmium
FDA (Fluorescein Diacetate)
495
520
Blue Diode
Flazo Orange
375-530
612
Argon-Ion
Fluorescamine
390
460
Blue Diode
Fluorescein
494
518
Argon-Ion
Fluorescein Isothiocyanate (FITC)
490
525
Argon-Ion
Fluo-3
485
503
Argon-Ion
Fluo-4
494
516
Argon-Ion
FluoroEmerald
494
520
Argon-Ion
FluoroGold (Hydroxystilbamidine)
323 (Low pH)
368 (High pH)
408 (Low pH)
565 (High pH)
Helium-Cadmium
FluoroJade
425
510
Blue Diode
FluoroRuby
555
582
Krypton-Argon
FluorX
494
520
Argon-Ion
FM1-43
479
598
Argon-Ion
FM4-64
515
640
Argon-Ion
Formaldehyde Induced Fluorescence
405
435
Blue Diode
Fura-2
363 (Low [Ca])
335 (High [Ca])
512 (Low [Ca])
505 (High [Ca])
Helium-Cadmium
Fura Red
472 (Low [Ca])
436 (High [Ca])
657 (Low [Ca])
637 (High [Ca])
Argon-Ion
Genacryl Brilliant Red B
520
590
Argon-Ion
Genacryl Brilliant Yellow 10GF
430
485
Blue Diode
Genacryl Pink 3G
470
583
Argon-Ion
Genacryl Yellow 5GF
430
475
Blue Diode
GFP
(Green Fluorescent Protein)
395, 475
510
Blue Diode
Gloxalic Acid
405
460
Blue Diode
Granular Blue
355
425
Helium-Cadmium
Haematoporphyrin
530-560
580
Green He-Ne
Hoechst 33258, 33342
(Bound to DNA)
352
461
Helium-Cadmium
3-Hydroxypyrene-5,8,10-TriSulfonic Acid
403
513
Blue Diode
7-Hydroxy-4-methylcoumarin
360
455
Helium-Cadmium
5-Hydroxy-Tryptamine (5-HT)
380-415
520-530
Blue Diode
1,5-IAEDANS
336
482
Helium-Cadmium
Indo-1
350
405-482
Helium-Cadmium
Indodicarbocyanine
644
665
Krypton-Argon
Intrawhite Cf (Liquid)
360
430
Helium-Cadmium
JC-1
514
529
Argon-Ion
6-JOE
525
555
Argon-Ion
JOJO-1, JO-PRO-1
529
545
Argon-Ion
Leucophor PAF
370
430
Argon-Ion
Leucophor SF
380
465
Argon-Ion
Leucophor WS
395
465
Blue Diode
Lissamine Rhodamine B200 (RD200)
575
595
Krypton-Argon
LOLO-1, LO-PRO-1
565
579
Krypton-Argon
Lucifer Yellow CH
425
528
Blue Diode
Lucifer Yellow VS
430
535
Blue Diode
LysoSensor Blue DND-192, DND-167
374
425
Argon-Ion
LysoSensor Green DND-153, DND-189
442
505
Argon-Ion
LysoSensor Yellow/Blue
384 (Low pH)
329 (High pH)
540 (Low pH)
440 (High pH)
Helium-Cadmium
LysoTracker Blue
373
422
Argon-Ion
LysoTracker Blue/White
466
536
Argon-Ion
LysoTracker Green
504
511
Argon-Ion
LysoTracker Red
577
590
Krypton-Argon
LysoTracker Yellow HCK-123
465
535
Green He-Ne
Magdala Red
524
600
Argon-Ion
Magnesium Green
506
531
Argon-Ion
Magnesium Orange
550
575
Green He-Ne
Marina Blue
362
459
Argon-Ion
Maxilon Brilliant Flavin 10 GFF
450
495
Argon-Ion
Maxilon Brilliant Flavin 8 GFF
460
495
Argon-Ion
Merocyanine 540
500
572
Argon-Ion
4-Methylumbelliferone
360
449
Helium-Cadmium
Mithramycin
450
570
Argon-Ion
MitoFluor Green
490
516
Argon-Ion
MitoFluor Red 589
588
622
Yellow He-Ne
MitoFluor Red 594
598
630
Orange He-Ne
MitoFluor Far Red 680
680
700
Red Diode
MitoTracker Green FM
490
516
Argon-Ion
MitoTracker Orange CMTMRos
554
576
Krypton-Argon
MitoTracker Red CMXRos
579
599
Krypton-Argon
MitoTracker Red 580
581
644
Krypton-Argon
MitoTracker Deep Red 633
644
665
Krypton-Argon
MPS (Methyl Green Pyronine Stilbene)
364
395
Argon-Ion
Monochlorobimane
380
461
Blue Diode
Nano Orange
485
590
Argon-Ion
NBD
450
530
Argon-Ion
Nile Red
515-530
525-605
Argon-Ion
Nitrobenzoxadidole
460-470
510-650
Argon-Ion
Noradrenaline
340
490-520
Helium-Cadmium
Nuclear Fast Red
289-530
580
Argon-Ion
Nuclear Yellow
365
495
Argon-Ion
Nylosan Brilliant Flavin E8G
460
510
Argon-Ion
Olivomycin
430
545
Blue Diode
OPT (o-Phthalaldehyde)
340
455
Helium-Cadmium
Oregon Green 488
496
524
Argon-Ion
Oregon Green 500
503
522
Argon-Ion
Oregon Green 514
511
530
Argon-Ion
Pacific Blue
410
455
Blue Diode
Pararosaniline (Feulgen)
570
625
Krypton-Argon
PE-B (Phycoerythrin B)
545
576
Green He-Ne
PE-R (Phycoerythrin R)
490, 565
578
Argon-Ion
Phorwite AR (Solution)
360
430
Helium-Cadmium
Phorwite BKL
370
430
Argon-Ion
Phorwite Rev
380
430
Argon-Ion
Phorwite RPA
375
430
Argon-Ion
Phosphine 3R
465
565
Argon-Ion
Phosphine R
480-565
578
Argon-Ion
Phycocyanin C
620
648
Orange He-Ne
Phycocyanin R
618
642
Orange He-Ne
PKH26
551
567
Green He-Ne
Pontochrome Blue Black
535-553
605
Green He-Ne
POPO-1, PO-PRO-1
434
456
Blue Diode
POPO-3, PO-PRO-3
534
572
Green He-Ne
Primuline O
410
550
Blue Diode
Procion Yellow
470
600
Argon-Ion
Propidium Iodide
536
617
Green He-Ne
PyMPO
415
570
Blue Diode
Pyrene
345
378
Helium-Cadmium
Pyronin Y
540
570
Green He-Ne
Pyronine
410
540
Blue Diode
Pyronine B
540-590
560-650
Krypton-Argon
Pyrozal Brilliant Flavin 7GF
365
495
Argon-Ion
Qdot 525
350-450
525
Blue Diode
Qdot 525
350-525
565
Blue Diode
Qdot 585
350-550
585
Blue Diode
Qdot 605
350-575
605
Blue Diode
Qdot 655
350-600
655
Blue Diode
Quinacrine Mustard
423
503
Blue Diode
Resorufin
570
585
Krypton-Argon
RH 414
532
716
Argon-Ion
Rhod-2
550
571
Green He-Ne
Rhodamine 110
496
520
Argon-Ion
Rhodamine 123
511
534
Argon-Ion
Rhodamine 5 GLD
470
565
Argon-Ion
Rhodamine 6G
526
555
Argon-Ion
Rhodamine B
540
625
Green He-Ne
Rhodamine B 200
523-557
595
Green He-Ne
Rhodamine B Extra
550
605
Green He-Ne
Rhodamine BB
540
580
Green He-Ne
Rhodamine BG
540
572
Green He-Ne
Rhodamine Green
502
527
Argon-Ion
Rhodamine Red
570
590
Krypton-Argon
Rhodamine WT
530
555
Argon-Ion
Rhodol Green
499
525
Argon-Ion
RITC (Rhodamine B Isothiocyanate)
570
595
Krypton-Argon
Rose Bengal
540
550-600
Green He-Ne
ROX (Carboxy-X-Rhodamine)
574
602
Krypton-Argon
SBFI
334
525
Helium-Cadmium
Serotonin
365
520-540
Argon-Ion
Sevron Brilliant Red 2B
520
595
Argon-Ion
Sevron Brilliant Red 4G
500
583
Argon-Ion
Sevron Brilliant Red B
530
590
Argon-Ion
Sevron Orange
440
530
Blue Diode
Sevron Yellow L
430
490
Blue Diode
SITS (Stilbeneisothiosulphonic Acid)
337
436
Helium-Cadmium
Sodium Green
507
535
Argon-Ion
Spectrum Aqua
433
480
Blue Diode
Spectrum Green
497
524
Argon-Ion
Spectrum Orange
559
588
Krypton-Argon
Spectrum Red
604
624
Orange He-Ne
Stilbene
335
440
Helium-Cadmium
Sulforhodamine 101
586
605
Yellow He-Ne
Sulforhodamine B
565
586
Krypton-Argon
Sulforhodamine G
529
548
Argon-Ion
SYTOX Blue
444
480
Blue Diode
SYTOX Green
504
523
Argon-Ion
SYTOX Orange
547
570
Green He-Ne
TAMRA (Carboxytetramethylrhodamine)
542
568
Green He-Ne
Tetracycline
390
560
Blue Diode
Texas Red
596
615
Yellow He-Ne
Thiazine Red R
510
580
Argon-Ion
Thiazole Orange
453
480
Argon-Ion
Thioflavin S
430
550
Blue Diode
Thioflavin TCN
350
460
Helium-Cadmium
Thiolyte
370-385
477-484
Argon-Ion
Tinopol CBS
390
430
Blue Diode
TOTO 1, TO-RRO-1
514
533
Argon-Ion
TOTO 3, TO-PRO-3
642
661
Krypton-Argon
TRITC
557
576
Krypton-Argon
True Blue
365
420-430
Argon-Ion
Ultralite
656
678
Krypton-Argon
Uranine B
420
520
Blue Diode
Uvitex SFC
365
435
Argon-Ion
X-Rhodamine
580
605
Krypton-Argon
Xylenol Orange
546
580
Green He-Ne
XRITC (X-Rhodamine Isothiocyanate)
582
601
Krypton-Argon
YOYO-1, YO-PRO-1
491
509
Argon-Ion
YOYO-3, YO-PRO-3
612
631
Orange He-Ne
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